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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Stathmin inhibits proliferation and differentiation of dental pulp stem cells via sonic hedgehog/Gli
doi: 10.1111/jcmm.13621
Figure Lengend Snippet: Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) fluorescence in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
Article Snippet: Then, the cell cycle phases G0/G1, S and G2/M were analysed using a
Techniques: Expressing, Transduction, shRNA, Transfection, Fluorescence, Western Blot, Real-time Polymerase Chain Reaction