flow cytometer data analyses Search Results


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Becton Dickinson fluorescence activated flow cytometer cell analyser bd falcon
Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) <t>fluorescence</t> in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
Fluorescence Activated Flow Cytometer Cell Analyser Bd Falcon, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc flow cytometer data analysis software cytoflex
Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) <t>fluorescence</t> in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
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Becton Dickinson flow cytometer becton–dickinson facscan analyser
Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) <t>fluorescence</t> in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
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Becton Dickinson flow cytometer analyser
Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) <t>fluorescence</t> in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
Flow Cytometer Analyser, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson automated flow cytometer facs-analyser
Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) <t>fluorescence</t> in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
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Becton Dickinson flow cytometer with a data handler cellquest software program
Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) <t>fluorescence</t> in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate
Flow Cytometer With A Data Handler Cellquest Software Program, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) fluorescence in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate

Journal: Journal of Cellular and Molecular Medicine

Article Title: Stathmin inhibits proliferation and differentiation of dental pulp stem cells via sonic hedgehog/Gli

doi: 10.1111/jcmm.13621

Figure Lengend Snippet: Expression of Stathmin in hDPSCs and lentivirus infections of hDPSCs. A, B, Expression of Stathmin in the cytomembrane and cytoplasm of hDPSCs (scale bar = 20 μm). C, D, At 72 h after gene transduction, both shRNA‐Stathmin‐ and shRNA‐Ctrl‐transfected hDPSCs grew well, and green fluorescent protein (GFP) fluorescence in the hDPSCs confirmed the transfection efficiency (scale bar = 100 μm). E, F, Western blot analysis showing that the Stathmin protein band in the shRNA‐Stathmin group was significantly weaker than that in the shRNA‐Ctrl group. G, Real‐time PCR showed that the expression levels of Stathmin messenger RNA were lower in the shRNA‐Ctrl group than in the shRNA‐Stathmin group ( ***P < .001). Each experiment was repeated in triplicate

Article Snippet: Then, the cell cycle phases G0/G1, S and G2/M were analysed using a fluorescence activated flow cytometer cell analyser (BD Falcon, San Jose, USA).

Techniques: Expressing, Transduction, shRNA, Transfection, Fluorescence, Western Blot, Real-time Polymerase Chain Reaction